The effect of sample handling and preparation on the iron binding polyphenolic content of browse

Citation

Brooks M, Hunt A, Ward A. 2003. The effect of sample handling and preparation on the iron binding polyphenolic content of browse. In Ward A, Brooks M, Maslanka M, Eds. Proceedings of the Fifth Conference on Zoo and Wildlife Nutrition, AZA Nutrition Advisory Group, Minneapolis, MN.

Abstract

The objective of this study was to determine feasible handling methods for accurate analysis of iron binding polyphenolics. Seven methods were tested with different combinations of both onsite handling and laboratory preparation. In these methods, on-site collection included freezing the samples with liquid nitrogen or dry ice or collecting the samples fresh and returning them to the lab. Once in the lab, samples were either ground immediately with liquid nitrogen, stored in the freezer, or placed in a drying oven at 60C. All dried samples were then ground in a Wiley Mill while all other samples were ground with liquid nitrogen. An iron binding assay was then used to determine total iron binding polyphenolic levels and the different processing methods were compared. Methods that involved freezing the samples tended to result in higher concentrations within most plant species versus the methods that used dry samples.

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