Use of a novel iron chelator (HBED) in black rhinoceros

Black rhinoceroses (Diceros bicornis) are critically endangered and are compromised by iron overload under human care (Paglia and Tsu, 2012; Molenaar et al., 2008). With the goal of safely reducing iron absorption in the gastrointestinal tract of affected and susceptible individuals, we investigated oral administration of the iron chelator, N,N-bis(2-hydroxybenzyl)ethylenediamine-N,N–diacetic acid (HBED) to 2.1 black rhinoceros. We expected HBED administration...

A retrospective investigation of the prevalence and significance of hemosiderosis in captive pinnipeds

Iron is a trace element required for the synthesis of haemoglobin and a number of energetic reactions. In most species, iron uptake is primarily regulated by the absorption of iron and protein-addition conversion to ferritin in the mucosal layers of the intestines and the sloughing of mucosal layers when iron levels are replete. However, if this system becomes imbalanced as...

Iron deficiency anemia in captive Malayan tapir calves (Tapirus indicus)

Subclinical iron deficiency anemia was diagnosed in a captive neonatal female Malayan tapir (Tapirus indicus) through blood samples obtained as part of an in-house training program for venipuncture. Routine blood testing performed at day 2 of age was within in-house and ISIS normal values for this species. Microcytic hypochromic anemia (HCT = 16 %; MCV = 38.4 fL; MCH =...

Dietary treatment of iron storage disease in captive birds of paradise (Paradisaea raggiana)

Elevated serum iron parameters were lowered through dietary manipulation in captive Bird of Paradise (Paradisaea raggiana) using a modification of previously published low-iron diets. Study birds were part of a captive breeding program consisting of two males and one female, captive born, 3.5 to 9 years of age. Serum iron, total iron binding capacity (TIBC), percent transferrin saturation, body weight,...

Determination of tannin levels in various plants and their possible effect on iron chelation in lemurs

Lemurs are one of several species that can suffer from excess iron accumulation in tissues, termed hemosiderosis. The more severe form, hemochromatosis, develops when hemosiderosis is accompanied by functional or morphologic evidence of iron toxicity and may result in clinical disease and death. Organs most often affected include the liver, heart, spleen, and gastrointestinal tract. The pathology of this problem/disease...

The effect of length of cold storage on secondary plant compounds in browse for captive black rhinoceros (Diceros bicornis)

The endangered black rhinoceros (Diceros bicornis) suffers from a high rate of mortality in captivity due to conditions such as hemosiderosis and hemolytic anemia. Supplementation of the diet with native browse species rich in tannins and other iron-binding polyphenolics has been proposed as a dietary strategy for reducing iron absorption and accumulation in this population. This study examined the effect...

The effect of sample handling and preparation on the iron binding polyphenolic content of browse

The objective of this study was to determine feasible handling methods for accurate analysis of iron binding polyphenolics. Seven methods were tested with different combinations of both onsite handling and laboratory preparation. In these methods, on-site collection included freezing the samples with liquid nitrogen or dry ice or collecting the samples fresh and returning them to the lab. Once in...

Analysis of nectar replacement products and a case of iron toxicosis in hummingbirds

Twenty-five of 26 hummingbirds (Trochilidae) from 7 species previously maintained in an aviary on a commercial nectar substitute for 13 years, died over 3-month period. Gross necropsy was inconclusive; however, staining of liver and intestinal tissue showed high levels of Fe deposition. Analysis of separate bags (n=9) of the nectar product, with identical lot numbers, showed variation in Fe content...

Summary of mineral and iron binding polyphenolic plant compound levels in diets offered captive black rhinoceros (Diceros bicornis) in 3 zoos and 1 ranch in Texas

Hemosiderosis is commonly noted in captive black rhinoceros but not free-ranging animals. Animals held in a ranch setting, offered a diet containing at least 46% browse on a dry matter basis, do not experience the same health problems as animals in zoos. It is known that plants contain polyphenolic compounds that can bind iron and make it unavailable for absorption....

The effect of tannin on the in vitro solubilization of iron

The absorption of dietary iron can be greatly influenced by other constituents in the diet, such as ascorbic acid (vitamin C) and tannins. Ascorbic acid increases the bioavailability of iron by converting Fe3+ to Fe2+, while tannins can reduce the bioavailability of iron by binding to it. Captive herbivorous animals are often impacted by either the addition or absence of...

Trace element intake of Cercopithecinae

The Fe, Zn, Cu, Mn and Mo intake was determined in 5 species of Cercopithecinae kept in captivity. The feed dry matter contained up to three times higher concentrations of trace elements compared to humans’ mixed and vegetarian diets. Although the mean body mass of the animals only amounted to 8% of the body mass of humans, they had a...

Variation in serum ferritin, serum iron, total iron binding capacity (TIBC) and percent transferrin saturation in Northern Fur Seals: a retrospective study

Hemochromatosis is an excessive accumulation of iron in tissues, particularly liver, and is associated with the development of hepatic lesions. The term hemosiderosis is used when there are no toxic effects of the iron accumulation. Both conditions have been observed in free-ranging and captive specimens of many species (Lowensteine and Munson 1999). Histopathologic analysis of tissues obtained from two aged...

Hemochromatosis (Iron Storage Disease) in Fruit Bats

Six Egyptian fruit bats (Rousettus aegyptiacus) were either found dead or presented with chronic liver disease associated with accumulations of iron (Fe) within the liver as high as 2.3% dry weight. Two other fruit bat species, the Indian flying fox (Pteropus giganteus) and the grey- headed flying fox (Pteropus poliocephalus), also had elevated liver Fe levels but without clinical evidence...

Iron Storage Disease in Lemurs

A syndrome of excessive iron accumulation (hemosiderosis) was first recognized in lemurs as early as the 1960’s but descriptive reports of the condition were no t published until the 1980’s.1,4,11 The most well-known of these is a publication by Spelman et al. (1989) who reported 100% incidence of hemosiderosis in captive lemurs from the institution in her study.11 As a...