Soto AB. 2013. Blood Glucose levels in three species of agalychnis sp.. In Ward A, Coslik A, Mahan K, Treiber K, Reppert A, Maslanka M, Eds. Proceedings of the Tenth Conference on Zoo and Wildlife Nutrition, AZA Nutrition Advisory Group, Salt Lake City, UT.


In the normal animal, the homeostasic level of blood glucose is mantained by the equilibrium between glucose supply and removal, as a result of a finely balance system of hormonal interactions.1 Reference glucose values can vary depending of the sex, weight, management and feeding systems, among others.3 Several studies have analyzed blood parameters for frogs, however, no previous studies have been found for Agalychnis sp. in the wild. The goal of this study was to determine blood glucose levels in three frog species commonly kept in captivity, to evaluate its health and nutritional status. Twenty seven adult frogs (n=17 A. callidryas, n=5 A. spurrelli and n=5 A. annae) were collected from the wild. Morphometric measurements were taken (weight and length). Then the animals were anesthetized using a solution of isofluorane, with a dose of 0.03 ml/g of body weight, and blood samples were obtained from heart puncture. Glucose was determined using a portable Multicare in® kit. Glucose levels were 51.88, 47.60 and 54.33 mg/dl for A. callidryas, A. spurrelli and A. annae respectively. These values are similar to those found in Rana catesbeiana.2,3 Significant differences were found in A. spurrelli compared to the other species. Weights and lengths were 5.89, 6.98 and 6.46 g and 50.55, 53.77 and 58.00 mm for A. callidryas, A. spurrelli and A. annae. The study still continues determining other blood parameters used to evaluate nutritional status in Agalychnis frogs, and information will be used further to compare animal status in captivity.

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